GLK, active, GST tagged human

NA.32

GLK is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. GLK is upstream of the MEKK1 target and is stimulated by UV radiation and TNF- and can lead to the activation of the JNK signaling pathway. GLK can directly activate PKC-θ during TCR signaling and people with systemic lupus erythematosus show considerable enhanced GLK expression and activation of PKC-θ and the kinase IKK in T cells. GLK can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM.

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

After opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

human

expressed in baculovirus infected Sf9 cells

PRECISIO^®

≥70% (SDS-PAGE)

buffered aqueous glycerol solution

74-110 nmol/min·mg

~70 kDa

NM_003618

cell analysis

dry ice

−70℃

human ... MAP4K3(8491)

10 - Combustible liquids

WGK 1