MULTI-seq Lipid-Modified Oligos

200

liquid

sufficient for 100 sample(s) (100RXN kit is adequate for labelling 100 samples when using 1 μL of each LMO per sample)
sufficient for 30 sample(s) (30RXN kit is adequate for labelling 30 samples when using 1 μL of each LMO per sample)

50 μM (each)

multiplexing: suitable (Single Cell and Single Nucleus)

dry ice

−20℃

MULTI-seq, multiplexing using lipid-tagged indices, is a novel tool that facilitates multiplex single-cell and single-nucleus RNA sequencing1. This technology provides sample barcoding that is compatible with any cell type or nucleus containing an accessible plasma membrane and allows for streamlined, pooled single-cell sample processing.

The MULTI-seq Lipid Modified Oligos kit is comprised of a lignoceric amide-modified anchor DNA oligo solution and a palmitic amide-modified co-anchor DNA oligo solution. Together, these lipid-modified oligos embed into cell or nuclei membranes and provide a landing pad for DNA barcodes with a complementary 5′ sequence. The MULTI-seq Lipid Modified Oligos kit is intended for upstream sample preparation only, before pooling and single cell analysis.

NOTE: Unique barcode oligos are not included and must be purchased separately.

Barcode 3′ oligo design with poly-A tail for mRNA enrichment1:
5′-CCTTGGCACCCGAGAATTCCA-8-base index-A30-3′

Barcode oligo design for 5′ cDNA library synthesis:
5′-CCTTGGCACCCGAGAATTCCA-8-base index-CCCATATAAGAAA-3′

Custom barcode oligos may be ordered from SigmaAldrich.com/nextgenoligos. For minimal cross-contamination of barcode primers, order Next-Gen Sequencing Oligos with NGSO-Silver or NGSO-Gold quality.

Reagents Provided

• LMO001A Lignoceric Anchor with DNA Oligo
• LMO001B Palmitic Co-anchor with DNA Oligo

Each lipid-modified oligo is supplied at a concentration of 50 μM in water.

12 - Non Combustible Liquids

WGK 1