Anti-Dopamine D₅ Receptor Rabbit pAb

rabbit

100

serum

primary antibodies

polyclonal

lyophilized

preservative

rat, human

Calbiochem^®

OK to freeze

IgG

ambient

2-8℃

100 μL in Plastic ampoule

Anti-Dopamine D5 Receptor, rabbit polyclonal, recognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~49 kDa D₅ dopamine receptor protein.
Recognizes the dopamine D₅ receptor in rat brain. Does not cross-react with other dopamine receptors.

a synthetic peptide (LPPGSNGTAYC) corresponding to amino acids 2-10 of human dopamine D₅ receptor, conjugated to carrier protein
Human

ELISA (see comments)

Flow Cytometry (1:100, see application references)

Immunoblotting (1:200)

Immunohistochemistry (1:2500)

Toxicity: Standard Handling (A)

Undiluted serum.

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Positive Control
Rat brain

McKenna, F., et al. 2002. J. Neuroimmunol.132, 34.
For immunohistochemistry, a 1:2500 dilution of the reconstituted antiserum demonstrated staining of specific cells in various regions of rat brain sections, including Cortex layer 5 and parafascicular nucleus. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

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10 - Combustible liquids

WGK 2