7-Amino-4-methylcoumarin-CAS 26093-31-2-Calbiochem

C₁₀H₉NO₂

175.18

MFCD00006868

Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.
Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.

Primary Target
Detection of proteolytic enzyme activity
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Irritant (B)

Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.

Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

yellow

DMSO: 10 mg/mL
acetone: 10 mg/mL
DMF: soluble

λ_ex 365-380 nm
λ_em 430-460 nm

ambient

2-8℃

1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3

GLNDAGDHSLMOKX-UHFFFAOYSA-N

10 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 3