Calpain Substrate II, Fluorogenic-CAS 94367-20-1-Calbiochem

C₂₉H₃₁N₃O₇

533.57

MFCD00057900

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.
Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

Product does not compete with ATP.
Reversible: no
Primary Target
Calpain -1 and -2
Cell permeable: no

Toxicity: Standard Handling (A)

Suc-Leu-Tyr-AMC

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (TLC)

solid

Calbiochem^®

OK to freeze
desiccated
protect from light

off-white

DMSO: soluble

λ_ex ~380  nm
λ_em ~460 nm

ambient

−20℃

1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1

VSWNAJAOJZXWBM-FTJGSZSVSA-N

25 mg in Plastic ampoule

11 - Combustible Solids

WGK 3